Course Information


Course Information
Course Title Code Semester L+U Hour Credits ECTS
BASIC RESEARCH TECHNIQUES II (APPL.) 56904015 0 + 6 3.0 6.0

Prerequisites None

Language of Instruction Turkish
Course Level Graduate Degree
Course Type Compulsory
Mode of delivery presentation, homework, experiment,
Course Coordinator
Instructors
Assistants
Goals To have knowledge about the basic principles and methods of cell culture, To practice cell isolation, culture, identification and cryopreservation techniques, To be able to work correctly, safely and successfully in cell culture laboratory
Course Content Safe working principles in laboratories and cell culture laboratories; Learning the principles and application methods of asepsis and sterilization in detail.
Learning Outcomes 1) Learning basic research techniques
2) Basic making cell culture
3) Use the basic devices used in the laboratory

Weekly Topics (Content)
Week Topics Teaching and Learning Methods and Techniques Study Materials
1. Week How about a cell culture lab. Question Answer; Demonstration
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
2. Week Safe working principles in laboratories and cell culture laboratories; Aseptic and sterilization principles and application methods Demonstration
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
3. Week Cell culture laboratory, use of related device-equipment
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
4. Week Factors affecting cell culture Demonstration
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
5. Week Characteristics of cell culture types and characteristics (primer culture, cell lines, suspension culture, adherent culture, cancer cells) Question Answer
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
6. Week Basic processes in cell culture: preparation of food, cell planting, nutrient replacement, subculture transition, cell counting, passaging, contamination control, culture maintenance and application Question Answer
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
7. Week Basic processes in cell culture: preparation of food, cell planting, nutrient replacement, subculture, cell counting, passaging, contamination control, culturing and monitoring.
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
8. Week Basic processes in cell culture: preparation of food, cell planting, nutrient replacement, subculture, cell counting, passaging, contamination control, culturing and monitoring. Question Answer
Colloquium
 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
9. Week Isolation of multipotent stromal cells from rat bone marrow, identification of primer culture and cells Discussion
Colloquium
Project Based Learning 		Report (Including Preparation and presentation Time) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
10. Week Isolation of multipotent stromal cells from rat bone marrow, identification of primer culture and cells
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
11. Week Isolation of multipotent stromal cells from rat bone marrow, identification of primer culture and cells
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
12. Week Principles of cryopreservation; Cell freezing, thawing and recultivation
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
13. Week GMP laboratory cell culture, large-scale production processes
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
14. Week GMP laboratory cell culture, large-scale production processes
Colloquium
Project Based Learning 		Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)

Sources Used in This Course
Recommended Sources
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Freshney, R.I., 6th Ed., 2010, Wiley. 2. Cytotechnology Journal. ISSN: 1573-0778, Springer.
Cytotechnology Journal. ISSN: 1573-0778, Springer.
Dersle İlgili Ulusal ve Uluslararası Tüm Kaynaklar

Relations with Education Attainment Program Course Competencies
Program RequirementsContribution LevelDK1DK2DK3
PY15000
PY25000
PY35555
PY45555

*DK = Course's Contrubution.
0 1 2 3 4 5
Level of contribution None Very Low Low Fair High Very High
.

ECTS credits and course workload
Event Quantity Duration (Hour) Total Workload (Hour)
Course Duration (Total weeks*Hours per week) 16 4
Work Hour outside Classroom (Preparation, strengthening) 4 4
Homework 4 4
Presentation (Including Preparation Time) 4 4
Project (Including Preparation and presentation Time) 4 4
Report (Including Preparation and presentation Time) 4 4
Activity (Web Search, Library Work, Trip, Observation, Interview etc.) 2 2
Practice (Teaching Practice, Music/Musical Instrument Practice , Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice) 2 2
Seminar 1 1
Internship 2 2
Quiz 1 1
Time to prepare for Quiz 1 1
Final Exam 1 1
Time to prepare for Final Exam 1 1
1 1
2 2
2 2
1 1
1 1
2 2
1 1
1 1
Dönem İçi Notu (DİN) 1 1
Dönem Sonu Sınavı (DSS) 1 1
Total Workload
Total Workload / 30 (s)
ECTS Credit of the Course
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Course Information
  • Course Information
  • Weekly Topics (Content)
  • Sources Used in This Course
  • Relations with Education Attainment Program Course Competencies
  • ECTS credits and course workload