Course Information


Course Information
Course Title Code Semester L+U Hour Credits ECTS
BASIC BIOTECHNOLOGY TECHNIQUES AND APPLICATIONS 400100711150 2 + 1 3.0 6.0

Prerequisites None

Language of Instruction Turkish
Course Level Graduate Degree
Course Type Compulsory
Mode of delivery
Course Coordinator
Instructors Demet CANSARAN DUMAN
Assistants
Goals The objective of this course is to teach the basic laboratory techniques used in biotechnology and molecular biology to graduate students.
Course Content The contents of the course starts with purification of DNA, RNA and protein from a wide variety of organisms and analyse their integrity and quality. DNA sequencing, PCR, qRT-PCR, cDNA synthesis, PAGE, ELISA, and many hybridization and chromatography based techniques were also included.
Learning Outcomes 1) Students will be able to demonstrate their knowledge of basic experimental techniques used in molecular biological studies.
2) Students gain the ability to perform molecular biological techniques to analyse DNA, RNA and proteins
3) Students will be able to comprehend and select correct molecular biological techniques to solve a problem
4) Students will be able to analyse and criticise the experimental results

Weekly Topics (Content)
Week Topics Teaching and Learning Methods and Techniques Study Materials
1. Week DNA isolation, determination of quantity and quality of DNA Lecture; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
2. Week PCR, types of PCR Lecture; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
3. Week Agarose gel electrophoresis Lecture; Question Answer; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
4. Week RNA isolation, determination of quantity and quality of RNA Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
5. Week Synthesis of cDNA, RT-PCR Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
6. Week qRT-PCR Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
7. Week Southern blot, Northern blot Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
8. Week Protein isolation, determination of quantity and quality of protein Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
9. Week PAGE Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
10. Week Western blot Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
11. Week ELISA Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
12. Week Purification techniques and chromatography Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
13. Week Capillary electrophoresis Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
14. Week DNA sequence analysis Lecture; Question Answer; Problem Solving; Discussion; Demonstration

 Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)

Sources Used in This Course
Recommended Sources
Green, M.R., Sambrook, J., 2012. Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume set, 4th edition. ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Miller, H., Witherow, D.S., Carson, S., 2011. Molecular Biology Techniques, Third Edition: A Classroom Laboratory Manual, 3 edition. ed. Academic Press, Oxford ; Waltham, MA.

Relations with Education Attainment Program Course Competencies
Program RequirementsContribution LevelDK1DK2DK3DK4
PY150000
PY250000
PY355555
PY455555

*DK = Course's Contrubution.
0 1 2 3 4 5
Level of contribution None Very Low Low Fair High Very High
.

ECTS credits and course workload
Event Quantity Duration (Hour) Total Workload (Hour)
Course Duration (Total weeks*Hours per week) 14 4
Work Hour outside Classroom (Preparation, strengthening) 14 1.5
Homework 4 2
Report (Including Preparation and presentation Time) 4 3
Activity (Web Search, Library Work, Trip, Observation, Interview etc.) 6 1
Practice (Teaching Practice, Music/Musical Instrument Practice , Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice) 14 3.5
Seminar 2 1
Quiz 4 1
Time to prepare for Quiz 4 2
Total Workload
Total Workload / 30 (s)
ECTS Credit of the Course
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Course Information
  • Course Information
  • Weekly Topics (Content)
  • Sources Used in This Course
  • Relations with Education Attainment Program Course Competencies
  • ECTS credits and course workload