Course Information


Course Information
Course Title Code Semester L+U Hour Credits ECTS
BASIC BIOTECHNOLOGICAL METHODS-I 23564023 2 + 0 3.0 8.0

Prerequisites None

Language of Instruction English
Course Level Graduate Degree
Course Type Compulsory
Mode of delivery
Course Coordinator
Instructors
Assistants
Goals The course aim is to taught the student basic biotechnological and molecular biological techniques
Course Content The objective of this course is to teach the basic laboratory techniques used in biotechnology and molecular biology to graduate students. The contents of the course starts with purification of DNA, RNA and protein from a wide variety of organisms and analyse their integrity and quality. DNA sequencing, PCR, qRT-PCR, cDNA synthesis, AGE, PAGE, ELISA, and many hybridization and chromatography based techniques were also included.
Learning Outcomes 1) The students will gain knowledge on basic experimental techniques used in molecular biology studies
2) The students will learn how to technically approach molecular biology studies
3) The students will learn how to troubleshoot the experiments.

Weekly Topics (Content)
Week Topics Teaching and Learning Methods and Techniques Study Materials
1. Week DNA: From isolation to the determination of quality and quantity Lecture; Question Answer
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
2. Week PCR Lecture; Problem Solving
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
3. Week Agarose gel electrophoresis Lecture; Question Answer; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
4. Week RNA: From isolation to the determination of quality and quantity Lecture; Question Answer; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
5. Week cDNA syntesis and RT-PCR Lecture; Question Answer; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
6. Week Real time PCR Lecture; Question Answer; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
7. Week Southern and Northern Blot Analyses Lecture; Question Answer; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
8. Week Protein: From isolation to the determination of quality and quantity Lecture; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
9. Week Polyacrylamide Gel Electrophoresis Lecture; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
10. Week Western Blot Lecture; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
11. Week ELISA Lecture; Problem Solving; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
12. Week Purification techniques and chromatography Lecture; Problem Solving; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
13. Week Capillary Electrophoresis Lecture; Problem Solving; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
14. Week DNA Sequencing Analysis Lecture; Discussion
Colloquium
Problem Based Learning 		Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)

Sources Used in This Course
Recommended Sources
Barker, Kathy. 1998. At the bench: a laboratory navigator. New York: Cold Spring Harbor Laboratory Press.
Green, M.R., Sambrook, J., 2012. Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume set, 4th edition. ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Miller, H., Witherow, D.S., Carson, S., 2011. Molecular Biology Techniques, Third Edition: A Classroom Laboratory Manual, 3 edition. ed. Academic Press, Oxford ; Waltham, MA.

Relations with Education Attainment Program Course Competencies
Program RequirementsContribution LevelDK1DK2DK3
PY15000
PY25555
PY35555
PY45000

*DK = Course's Contrubution.
0 1 2 3 4 5
Level of contribution None Very Low Low Fair High Very High
.

ECTS credits and course workload
Event Quantity Duration (Hour) Total Workload (Hour)
Course Duration (Total weeks*Hours per week) 14 4
Work Hour outside Classroom (Preparation, strengthening) 14 3
Homework 4 4
Report (Including Preparation and presentation Time) 14 2
Activity (Web Search, Library Work, Trip, Observation, Interview etc.) 14 1
Practice (Teaching Practice, Music/Musical Instrument Practice , Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice) 14 4
Seminar 2 1
Quiz 4 1
Time to prepare for Quiz 4 2
Time to prepare for Midterm Exam 1 10
Final Exam 1 2
Total Workload
Total Workload / 30 (s)
ECTS Credit of the Course
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Course Information
  • Course Information
  • Weekly Topics (Content)
  • Sources Used in This Course
  • Relations with Education Attainment Program Course Competencies
  • ECTS credits and course workload