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Course Information

Course Information

Course Title	Code	Semester	L+U Hour	Credits	ECTS
PCR APPLICATIONS IN AQUACULTURE	801700805130		3 + 0	3.0	10.0

Prerequisites

None

Language of Instruction	Turkish
Course Level	Graduate Degree
Course Type	Compulsory
Mode of delivery	Presentatin and Discussion
Course Coordinator
Instructors
Assistants
Goals	This course starts with the historical development process of the polymerase chain reaction (PCR) and explaining the differences with the current direct PCR protocols and related common PCR based applications used in aquaculture and explaining the kinetics of PCR.
Course Content	Basic concepts such as optimization of the PCR, primer design, analysis of PCR products, sequencing, in vitro expression, purification and cloning will be discussed. Also, the Real Time PCR (RT-PCR), normalization and analysis of RT-PCR data will be given within contents of this course. After the basic concepts, most common PCR based techniques used in aquaculture area, like species identification, DNA barcoding, metabarcoding, environmental DNA and designing minibarcodes will be discussed using examples from latest studies.
Learning Outcomes	1) "Comprehension of general theory of PCR based molecular techniques " 2) Understanding of the interactions between molecular biology and its applications in aquaculture 3) Conceive a general approach to PCR based molecular techniques in aquaculture