Course Information


Course Information
Course Title Code Semester L+U Hour Credits ECTS
INTRODUCTION TO PLANT TISSUE CULTURE 800200715310 3 + 0 3.0 8.0

Prerequisites None

Language of Instruction Turkish
Course Level Graduate Degree
Course Type Compulsory
Mode of delivery Face to face
Course Coordinator
Instructors
Assistants
Goals To give information about the methods of producing new plants or products from tissues, organs, cells and cell parts separated (isolated) from plants, working principles in aseptic conditions, content of nutrient media, information and experience for preparation of medium, information about plant tissue culture usage areas for practice.
Course Content Definition, importance and history of plant tissue culture, establishment of a tissue culture laboratory, sterilization tehniques, disinfectans, preparation of the plant materials for the culture, contents of the plant nutrient medium and their preparation techniques, cultivation, transplantation, subcultures, incubation conditions, observations on the growth of the cultures, proliferation and rhizogenesis.
Learning Outcomes 1) Have knowledge on the principles of working on aseptic (sterile) conditions.
2) Have knowledge about selection of nutrient medium composition
3) Have knowledge in preparing nutrient medium
4) Has knowledge about tissue culture laboratory establishment and design
5) Have knowledge about explant types

Weekly Topics (Content)
Week Topics Teaching and Learning Methods and Techniques Study Materials
1. Week Explanation of plant tissue culture concept Lecture; Question Answer; Discussion

 Presentation (Including Preparation Time)
2. Week Terms and meanings used in plant tissue culture Lecture; Question Answer

 Presentation (Including Preparation Time)
3. Week Basics of plant tissue culture laboratory establishment and equipments Lecture; Question Answer; Discussion

 Presentation (Including Preparation Time) Activity (Web Search, Library Work, Trip, Observation, Interview etc.)
4. Week Structure and composition of nutrient media Lecture; Question Answer

 Presentation (Including Preparation Time)
5. Week Preparation of nutrient medium Lecture; Discussion; Demonstration

 Presentation (Including Preparation Time) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
6. Week Aseptic working conditions and sterilization Lecture; Question Answer

 Presentation (Including Preparation Time)
7. Week Midterm Question Answer
8. Week Explant types and their use Lecture; Discussion; Demonstration

 Presentation (Including Preparation Time)
9. Week Establishment of the culture, transplantation of the explants and considerations in the explant inoculation phase Lecture; Discussion

 Presentation (Including Preparation Time)
10. Week Problems and solutions in tissue culture Lecture; Discussion

 Presentation (Including Preparation Time)
11. Week The necessary conditions and practices in incubation stage of the cultures, culture types Lecture; Discussion

 Presentation (Including Preparation Time)
12. Week Acclimatization, problems and solutions Lecture; Discussion

 Presentation (Including Preparation Time)
13. Week Laboratory work and visits, excursion Question Answer; Discussion; Education Excursion

 Activity (Web Search, Library Work, Trip, Observation, Interview etc.) Practice (Teaching Practice, Music/Musical Instrument Practice, Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice)
14. Week Presentation of homeworks and discussion Lecture; Discussion; Demonstration

 Homework Presentation (Including Preparation Time) Report (Including Preparation and presentation Time)

Sources Used in This Course
Recommended Sources
Gürel, E., Babaoğlu, M., Özcan, S. 2001. Bitki Biyoteknolojisi ve Genetik İlkeler, Teknikler ve Uygulamalar. Selçuk Üniversitesi Vakfı Yayınları, ISBN : 9789756652046
Pierik, R.L.M., 1997. In Vitro Culture of Higher Plants, 4th edn., Kluwer Academic Publishers, Dordrecht, The Netherlands.
Smith, R.H. 2013. Plant Tissue Culture, Techniques and Experiments. 3th edn, Elsevier, 187 p.
Stewart, Jr., C. N. (Çeviri Editörü: H.A. Öktem, M. Yücel). 2012. Plant Biotechnology and Genetics: Principles, Techniques, and Applications (Bitki Biyoteknolojisi ve Genetiği), 1. Baskı, Nobel Akademik Yayıncılık

ECTS credits and course workload
Event Quantity Duration (Hour) Total Workload (Hour)
Course Duration (Total weeks*Hours per week) 14 3
Work Hour outside Classroom (Preparation, strengthening) 14 2
Homework 2 4
Presentation (Including Preparation Time) 2 4
Report (Including Preparation and presentation Time) 2 4
Activity (Web Search, Library Work, Trip, Observation, Interview etc.) 10 3
Practice (Teaching Practice, Music/Musical Instrument Practice , Statistics, Laboratory, Field Work, Clinic and Polyclinic Practice) 4 3
Seminar 2 3
Midterm Exam 1 2
Time to prepare for Midterm Exam 1 4
Final Exam 1 2
Time to prepare for Final Exam 1 10
1 2
2 4
2 4
1 4
8 8
Total Workload
Total Workload / 30 (s)
ECTS Credit of the Course
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Course Information
  • Course Information
  • Weekly Topics (Content)
  • Sources Used in This Course
  • ECTS credits and course workload